Decontamination of Ribonuclease (RNase) for Next Generation Sequencing

RNA library prep, sequencing, and PCR

Learn more about decontamination of ribonuclease

Decontamination of Ribonuclease (RNase) for Next Generation Sequencing

RNA library prep, sequencing, and PCR

UV LED technology offers a new method of decontamination of ribonuclease (RNase) for next-generation sequencing labs. High Intensity, UV decontamination of Ribonuclease enables researchers to save significant time and money while ensuring consistent, accurate results.

As scientists already know, laboratory contamination is an on-going problem for experiments run in Next-Generation Sequencing (NGS). Significant levels of ambient RNase contamination can occur in minutes. Contaminants such as RNase A are difficult to irreversibly inactivate in the absence of harsh chemicals.

Phoseon’s KeyPro™ decontamination system deally suits abs that require high fidelity RNA library prep, sequencing, and PCR. Utilizing Phoseon’s patented Semiconductor Light Matrix (SLM)™ technology enables the KeyPro system to increase lab productivity. Complete inactivation of laboratory contaminants, including the hard-to-kill RNase A, can be accomplished by the KeyPro system in under five minutes and at fraction of the cost of traditional methods. Load reagents and solvents and run your decontamination cycle just prior to adding sample.

Why Ribonuclease Contamination is a Problem for RNA Sequencing Labs
In a lab environment, the single most important aspect of RNA protocols is isolating and maintaining full length, un-degraded RNA for analysis or use as a reaction substrate. Hindering this process is the presence of RNase. Whether preparing total RNA libraries for Next Generation Sequencing (NGS) or looking at individual RNAs (iCLIP), degradation by RNases is a recurring laboratory handling issue requiring diverse cleaning methods.

Once a package of disposables is opened, the contents can become contaminated and no longer suitable for RNA work. Pipettes left out on the bench can accumulate dust and microbial contamination from the room air and need frequent re-cleaning. Cleaning surfaces and equipment with sprays and rinses can leave chemical residues, an additional type of contamination, which may interfere with downstream biochemical reactions.

Furthermore, repeated exposure to cleaning solutions or soaking may corrode metal or degrade plastic surfaces. How clean is clean enough? Clean enough occurs when you don’t need to repeat lengthy protocols because of degraded RNA. Even trace amounts of RNase have a big impact on RNA sequencing, due to its catalytic action. Not clean leads to time and money loss.

Decontamination of Ribonuclease

Learn more about KeyPro Decontamination system for next generation sequencing (NGS)