{"id":249,"date":"2018-12-29T22:15:53","date_gmt":"2018-12-30T06:15:53","guid":{"rendered":"https:\/\/phoseon.com\/?page_id=249"},"modified":"2022-07-25T12:55:00","modified_gmt":"2022-07-25T19:55:00","slug":"decontamination-of-ribonuclease-for-next-generation-sequencing","status":"publish","type":"page","link":"https:\/\/phoseon.com\/pt\/life-sciences\/life-sciences-applications\/decontamination\/decontamination-of-ribonuclease-for-next-generation-sequencing\/","title":{"rendered":"Descontamina\u00e7\u00e3o do Ribonuclease"},"content":{"rendered":"<section class=\"two-column-flex\">\n\t\t\t\t<div class=\"two-column-flex-container\">\n\t\t\t<div class=\"flex-item small-flex-item\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<img decoding=\"async\" alt=\"\" src=\"https:\/\/phoseon.com\/wp-content\/uploads\/2019\/01\/RNase_A_Activity_Update-640x427.jpg\" \/>\n\t\t\t\t\t\t\t\t\t\t\t\t<\/div>\n\t\t\t<div class=\"flex-item large-flex-item\">\n\t\t\t\t\t\t\t\t\t\t\t\t\t<p class=\"two-column-cta-image-content\"><p>A tecnologia UV LED oferece um novo m\u00e9todo de descontamina\u00e7\u00e3o de ribonuclease (RNase) para laborat\u00f3rios de sequenciamento de pr\u00f3xima gera\u00e7\u00e3o. A descontamina\u00e7\u00e3o UV de alta intensidade de Ribonuclease permite aos pesquisadores economizar tempo e dinheiro significativos, ao mesmo tempo em que garante resultados consistentes e precisos.<\/p>\n<p>Como os cientistas j\u00e1 sabem,\u00a0<a href=\"https:\/\/www.neb.com\/tools-and-resources\/usage-guidelines\/avoiding-ribonuclease-contamination\" target=\"_blank\" rel=\"noreferrer noopener\" aria-label=\"a contamina\u00e7\u00e3o do laborat\u00f3rio \u00e9 um problema cont\u00ednuo (abre em uma nova aba)\">a contamina\u00e7\u00e3o laboratorial \u00e9 um problema cont\u00ednuo<\/a>\u00a0para experimentos executados em Seq\u00fcenciamento de Pr\u00f3xima Gera\u00e7\u00e3o (NGS). N\u00edveis significativos de contamina\u00e7\u00e3o ambiental RNase podem ocorrer em minutos. Contaminantes como o RNase A s\u00e3o dif\u00edceis de inativar irreversivelmente na aus\u00eancia de produtos qu\u00edmicos agressivos.<\/p>\n<p>Foseon's\u00a0<a href=\"https:\/\/phoseon.com\/pt\/life-sciences\/life-sciences-applications\/decontamination\/keypro-microplate-uv-led-decontamination-system\/\">KeyPro\u2122 sistema de descontamina\u00e7\u00e3o<\/a>\u00a0deally se adapta aos ausentes que requerem prepara\u00e7\u00e3o de biblioteca de RNA de alta fidelidade, seq\u00fcenciamento e PCR. Utilizando o patenteado Phoseon's\u00a0<a href=\"https:\/\/phoseon.com\/pt\/life-sciences\/life-sciences-technology\/high-intensity-led-light-engines\/\">Matriz Semicondutora de Luz (SLM)\u2122 tecnologia<\/a>\u00a0permite que o sistema KeyPro aumente a produtividade do laborat\u00f3rio. A inativa\u00e7\u00e3o completa dos contaminantes de laborat\u00f3rio, incluindo o RNase A de dif\u00edcil elimina\u00e7\u00e3o, pode ser realizada pelo sistema KeyPro em menos de cinco minutos e por uma fra\u00e7\u00e3o do custo dos m\u00e9todos tradicionais. Carregue os reagentes e solventes e execute seu ciclo de descontamina\u00e7\u00e3o imediatamente antes de adicionar a amostra.<\/p>\n<\/p>\t\t\t<\/div>\n\t\t<\/div>\n\t<\/section>\n\n\n\n\n\n\n\n\n<p><strong>Por que a contamina\u00e7\u00e3o por Ribonuclease \u00e9 um problema para os laborat\u00f3rios de seq\u00fcenciamento de RNA<\/strong><br>Em um ambiente de laborat\u00f3rio, o aspecto mais importante dos protocolos de RNA \u00e9 isolar e manter o RNA completo, n\u00e3o degradado, para an\u00e1lise ou uso como substrato de rea\u00e7\u00e3o. O que dificulta este processo \u00e9 a presen\u00e7a do RNase. Seja preparando bibliotecas totais de RNA para o Seq\u00fcenciamento da Pr\u00f3xima Gera\u00e7\u00e3o (NGS) ou olhando para RNAs individuais (iCLIP), a degrada\u00e7\u00e3o por RNases \u00e9 um problema recorrente de manuseio de laborat\u00f3rio que requer diversos m\u00e9todos de limpeza. <\/p>\n\n\n\n<p>Uma vez aberto um pacote de descart\u00e1veis, o conte\u00fado pode se tornar contaminado e n\u00e3o mais adequado para o trabalho de RNA. As pipetas deixadas na bancada podem acumular poeira e contamina\u00e7\u00e3o microbiana do ar da sala e precisam ser limpas novamente com freq\u00fc\u00eancia. As superf\u00edcies e equipamentos de limpeza com sprays e enx\u00e1g\u00fces podem deixar res\u00edduos qu\u00edmicos, um tipo adicional de contamina\u00e7\u00e3o, que pode interferir nas rea\u00e7\u00f5es bioqu\u00edmicas a jusante. <\/p>\n\n\n\n<p>Al\u00e9m disso, a exposi\u00e7\u00e3o repetida a solu\u00e7\u00f5es de limpeza ou imers\u00e3o pode corroer o metal ou degradar as superf\u00edcies pl\u00e1sticas. Qu\u00e3o limpo \u00e9 limpo o suficiente? Limpo o suficiente ocorre quando voc\u00ea n\u00e3o precisa repetir protocolos longos por causa do RNA degradado. Mesmo quantidades vestigiais de RNase t\u00eam um grande impacto no seq\u00fcenciamento de RNA, devido \u00e0 sua a\u00e7\u00e3o catal\u00edtica. N\u00e3o limpo leva a perda de tempo e dinheiro. <\/p>\n\n\n\n<figure class=\"wp-block-image\"><img loading=\"lazy\" decoding=\"async\" width=\"1024\" height=\"683\" src=\"https:\/\/phoseon.com\/wp-content\/uploads\/2019\/01\/Next-Generation-Sequencing-Phoseon-Decontamination-1-1024x683.jpg\" alt=\"Descontamina\u00e7\u00e3o do Ribonuclease\" class=\"wp-image-2625\"\/><\/figure>\n\n\n\n<p><a href=\"https:\/\/phoseon.com\/pt\/life-sciences\/resources\/contact-life-sciences\/\">Saiba mais sobre o sistema de descontamina\u00e7\u00e3o KeyPro para o sequenciamento da pr\u00f3xima gera\u00e7\u00e3o (NGS)&nbsp;<\/a><\/p>\n\n\n\n<p><\/p>","protected":false},"excerpt":{"rendered":"<p>Why Ribonuclease Contamination is a Problem for RNA Sequencing LabsIn a lab environment, the single most important aspect of RNA [&hellip;]<\/p>","protected":false},"author":5,"featured_media":0,"parent":245,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v22.6 - https:\/\/yoast.com\/wordpress\/plugins\/seo\/ -->\n<title>Decontamination of Ribonuclease - Phoseon Technology<\/title>\n<meta name=\"description\" content=\"UV LED technology is a new method used in next-generation sequencing (NGS) for UV decontamination of ribonuclease (RNase). 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