去污研究已经确定,高辐照度的紫外线LED能成功地使DNA和RNA等生物分子失活。即使是像RNaseA这样的硬目标,也可以通过适当波长和强度的紫外线在几分钟内完全失活。
RNase的失活是测序和其他依赖RNA的研究的一个持续挑战。虽然人们早就知道紫外线可以使酶失活,但RNaseA特别难以失活,相当一部分在高压灭菌后仍有活性,因此必须使用DEPC处理或其他化学处理。维护无RNase的工作区域和表面是一个持续的、耗时的挑战,一个快速、有效的RNase灭活方法将是一个好处。我们的研究已经确定,紫外LED光源可以提供比标准汞紫外灯高得多的定向辐射功率,允许在更短的时间内提供更高的剂量(J/cm²)。
我们发现275nm和365nm波长的RNaseA灭活的效果之间的协同作用。 这表明,使用多个目标波长是控制和快速灭活蛋白质和酶的一个可行的路径。
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