Preparative (Prep) chromatography purifies sufficient quantities of a substance for further use, rather than analysis. Flash chromatography is a form of prep chromatography with lower pressure and much higher flow rate. Phoseon’s KeyViewTM UV detectors integrate well into both preparative chromatography and flash chromatography applications.
For preparative chromatography, the goal of the user is to determine analyte concentrations with high absolute absorption. Using solid state technology, KeyView detectors for liquid chromatography have the ability to increase light intensity to capture a greater dynamic range of high concentration analytes. With the multi-channel monitoring of only the relevant wavelengths, Phoseon’s detectors allow users to consistently quantify and isolate fractions of proteins and biomolecules. The near zero drift with KeyView gives users the confidence of highly reproducible results.
Absorbance peak spectrum for maximum linearity range using concentrated squid ink. 220 nm, 255 nm, 265 nm, 275 nm, 285 nm, and 307 nm